Contact
Intranet
SK
International
| Hybrid DNA-functionalized fibrils as nanostructured material for bioanalytical applications | |
| Hybrid DNA-functionalized fibrils as nanostructured material for bioanalytical applications | |
| Program: | Mobility |
| Project leader: | RNDr., Ing. Šipošová Katarína, PhD. |
| Duration: | 1.1.2024 – 31.12.2025 |
| Hyp4Amy – Ultrazvukom a magneticky indukovaná hypertermia ako liečebná modalita pre amyloidné ochorenia | |
| Ultrasound- and Magnetic-induced Hyperthermia as a Treatment Modality for Amyloid-related Diseases | |
| Program: | Bilateral – other |
| Project leader: | RNDr., Ing. Šipošová Katarína, PhD. |
| Annotation: | Nanoparticles (NPs), including those with magnetic properties, have attracted significant scientific interest due totheir applications in various fields of science and medicine. As a consequence, several promising treatmentprocedures have been developed, such as controlled and local drug delivery and release, photo- and sonodynamictherapy, and thermal therapy. NPs can also be used as biosensors or imaging contrast agents. Additionally, theyare being studied for their potential role in amyloidogenic diseases. Although great efforts have been made tounderstand the pathogenesis of these diseases and design effective therapy, there is still no treatment for amyloid -related diseases. A possible alternative non-pharmacological option for targeting cross -using energy generated by magnetic NPs as a result of applied magnetic field and/or ultrasound to disrupt largeamyloid structures (plaques) into smaller fragments. This proposal is aimed at basic as well as applied researchthat will help perfect the application of magnetic NPs in the treatment of amyloid-related diseases via thermal andultrasound-induced processes and enable tomography imaging of amyloid formation/decomposition. Efficaciousdrug/NPs deposition in the target site and operated activation of therapeutic and imaging agents reduce treatmenttime, while avoiding adverse damage and side effects resulting from systemic administration. The collaboration willnot only promote the bilateral exchange of knowledge, technology and experience, but also may accelerate thetranslation between biophysical/biochemical science and clinical study. |
| Duration: | 1.1.2024 – 31.12.2025 |
| Innovative water-soluble phytomaterial inhibitors for Alzheimer’s and Parkinson’s disease prevention | |
| Innovative water-soluble phytomaterial inhibitors for Alzheimer’s and Parkinson’s disease prevention | |
| Program: | Horizon 2020 |
| Project leader: | MUDr. Musatov Andrey, DrSc. |
| Duration: | 1.10.2022 – 28.2.2025 |
National
| SelfNano – Programovateľné samo-usporiadanie hybridných DNA-proteín nanosystémov pre kontrolovateľné viazanie a uvoľnovanie biologicky aktívnych látok | |
| Programmable self-organization of hybrid DNA-protein nanosystems for controlled binding and release of biologicals | |
| Program: | SRDA |
| Project leader: | RNDr., Ing. Šipošová Katarína, PhD. |
| Annotation: | Protein self-assembly is a process based on autonomous, non-covalent interactions between distinct building blocks without requirement of external energy sources. The possibility of chemical appending of functional ligands onto self-assembling peptides or proteins lays the foundation for developing new materials with unprecedented structural and functional features. Especially using sequence addressable DNAs, the synergistic combination into DNA-protein self-assembling systems, may lead to unique and sophisticated functional hybrid nanostructures, which are highly programmable and display remarkable features that create new opportunities to build materials on the nanoscale. Inspired by the unique ability of proteins to self-assemble into amyloid fibrils, we plan to use recombinant spider silk eADF4(C16) protein, insulin, Aβ peptide and lysozyme in order to demonstrate the versatility of the concept of DNA-assisted self-organization of higher-order fibrillar structures. We will explore two dynamic association modes, the temperature-controlled hybridization event of short overlapping DNA sequences and the highly specific DNA-aptamer-to-ligand binding controlled by the ligand affinity. Generally, we foresee the feasibility of the proposed nanofibrillar systems mate of DNA-protein hybrids for the construction of nanostructured materials in biomedical research for binding and release of biologically active agents, formation of multiple protein arrangements for efficient enzymatic cascades or even dyes positioning for efficient light harvesting systems. |
| Duration: | 1.7.2024 – 30.6.2028 |