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| SUPRA-SIGHT – Štúdium štruktúry a dynamiky supramolekulárnych amyloidných štruktúr jednomolekulovou mikroskopiou a modelovaním pomocou strojového učenia | |
| Exploring Protein Amyloid Superstructures and Dynamics Using Single-Molecule Microscopy with the Deep-Learning Modeling | |
| Program: | JRP |
| Project leader: | RNDr., Ing. Šipošová Katarína, PhD. |
| Annotation: | Protein self-assembly processes independent of external energy sources and unlimited in-dimensional scaling have become a very promising approach for developing new materials with unprecedented structural and functional features. The best examples of self-assembled materials can be found in nature, including functional and pathological amyloid fibrils. Motivated by this, the proposal is aimed at basic as well as applied research and is represented by two major goals: a) anti-amyloid study – focusing on pathological amyloid aggregation connected with neurodegenerative diseases that will optimize the application of newly prepared compounds/particles in the treatment of amyloid-related diseases and enable imaging of amyloid formation/decomposition; b) fabrication of protein superstructures by chemical appending of functional ligands onto self-assembling peptides or proteins lays the foundation for developing new materials with unprecedented structural and functional features.Therefore, the development of the single-molecule orientation and localization microscopy (SMOLM), together with the deep-learning modeling for the localization and reconstruction of SMOLM images will help rapidly perfect the high-content analysis of the structural-morphological features not only the pathological amyloid aggregates but also superstructures consisting of amyloid scaffolds and connected bio-structures (DNA) and ligands. Moreover, the single-molecule orientation and localization microscopy could significantly contribute to understanding not only the process of protein self-assembly but also the mechanism of the binding and release of biologically active agents, formation and reorganization of multiple protein arrangements upon interaction with biologically relevant ligands/molecules. |
| Duration: | 1.1.2025 – 31.12.2027 |
| AZCAI – Anti-amyloidná aktivita kompozitov na báze zeolitov a analýza so zobrazovaním vo vysokom rozlíšení a v reálnom čase | |
| Anti-amyloid activity of zeolite-based composites and analysis with real-time 3d super-resolution imaging | |
| Program: | JRP |
| Project leader: | RNDr., Ing. Šipošová Katarína, PhD. |
| Annotation: | A common feature associated with most of neurodegenerative diseases, including Alzheimer’s disease is the formation of extended, β-sheet rich amyloid fibrils. Today, amyloid-related diseases are incurable and the treatment is only symptomatic without feasibility to stop or substantially delay the progressive consequences of the diseases. Magnetic nano/micro-particles based on clinoptilolite-type of natural zeolite (CZ) jointly developed are expected to serve synergistic therapy approaches act as carriers for controlled drug delivery/release, imaging and local heating in biological systems, that can effectively decompose the amyloid-like fibrillar structures. The micro and mesopores of the natural zeolite can serve as containers for delivering various drugs to the target site to release. Magnetic CZ (MCZs) will improve drug delivery process, real-time monitoring of drug distribution surrounding a targeting side of tissue, as well as the subsequent effects of the therapeutics on the progression of diseases. In addition, fluorescent MCZs in combination with ultrasonic, magnetic or laser irradiation effects will provide hyperthermia and photoreaction to achieve both diagnosis and therapy. The Taiwanese research team has been deeply cultivated in temporal focusing multiphoton microscopy (TFMPM), which imaging frame rate can achieve up to a hundred hertz. We will use the deep learning method to improve the imaging frame rate for real-time biomedical analysis. Within this project, animal models (including genetic rodent models) will be utilized to develop a theranostic system for for inhibition and destruction of amyloid aggregates and super-resolution imaging of MZC induced amyloid aggregate inhibition/destruction effects by state-of-art temporally and spatially super-resolution 3D imaging technology. |
| Duration: | 1.1.2022 – 31.12.2024 |
| Zvýšenie Bioaktivity Nanočastíc Oxidu Céria | |
| Enhancement of Bioactivity of Cerium Oxide Nanoparticles | |
| Program: | Inter-academic agreement |
| Project leader: | MUDr. Musatov Andrey, DrSc. |
| Duration: | 1.1.2020 – 31.12.2021 |
National
| Nový pohľad na vplyv hydrofóbnych interakcií na tvorbu a stabilitu proteínových agregátov. Prepojenie na oxidačný stres. | |
| New Insight into the Role of Hydrophobic Interactions in Formation and Stability of Proteins Aggregates. Link to Oxidative Stress. | |
| Program: | VEGA |
| Project leader: | MUDr. Musatov Andrey, DrSc. |
| Duration: | 1.1.2021 – 31.12.2023 |
| Štúdium anti-oxidačnej a zosilnenej anti-amyloidnej aktivity nanočastíc céru pre biomedicínske aplikácie | |
| – | |
| Program: | DoktoGrant |
| Project leader: | RNDr. Garčárová Ivana |
| Duration: | 1.1.2021 – 31.12.2021 |