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| Hybrid DNA-functionalized fibrils as nanostructured material for bioanalytical applications | |
| Hybrid DNA-functionalized fibrils as nanostructured material for bioanalytical applications | |
| Program: | Mobility |
| Project leader: | RNDr., Ing. Šipošová Katarína, PhD. |
| Duration: | 1.1.2024 – 31.12.2025 |
| Innovative water-soluble phytomaterial inhibitors for Alzheimer’s and Parkinson’s disease prevention | |
| Innovative water-soluble phytomaterial inhibitors for Alzheimer’s and Parkinson’s disease prevention | |
| Program: | Horizon 2020 |
| Project leader: | MUDr. Musatov Andrey, DrSc. |
| Duration: | 1.10.2022 – 28.2.2025 |
| AZCAI – Anti-amyloidná aktivita kompozitov na báze zeolitov a analýza so zobrazovaním vo vysokom rozlíšení a v reálnom čase | |
| Anti-amyloid activity of zeolite-based composites and analysis with real-time 3d super-resolution imaging | |
| Program: | JRP |
| Project leader: | RNDr., Ing. Šipošová Katarína, PhD. |
| Annotation: | A common feature associated with most of neurodegenerative diseases, including Alzheimer’s disease is the formation of extended, β-sheet rich amyloid fibrils. Today, amyloid-related diseases are incurable and the treatment is only symptomatic without feasibility to stop or substantially delay the progressive consequences of the diseases. Magnetic nano/micro-particles based on clinoptilolite-type of natural zeolite (CZ) jointly developed are expected to serve synergistic therapy approaches act as carriers for controlled drug delivery/release, imaging and local heating in biological systems, that can effectively decompose the amyloid-like fibrillar structures. The micro and mesopores of the natural zeolite can serve as containers for delivering various drugs to the target site to release. Magnetic CZ (MCZs) will improve drug delivery process, real-time monitoring of drug distribution surrounding a targeting side of tissue, as well as the subsequent effects of the therapeutics on the progression of diseases. In addition, fluorescent MCZs in combination with ultrasonic, magnetic or laser irradiation effects will provide hyperthermia and photoreaction to achieve both diagnosis and therapy. The Taiwanese research team has been deeply cultivated in temporal focusing multiphoton microscopy (TFMPM), which imaging frame rate can achieve up to a hundred hertz. We will use the deep learning method to improve the imaging frame rate for real-time biomedical analysis. Within this project, animal models (including genetic rodent models) will be utilized to develop a theranostic system for for inhibition and destruction of amyloid aggregates and super-resolution imaging of MZC induced amyloid aggregate inhibition/destruction effects by state-of-art temporally and spatially super-resolution 3D imaging technology. |
| Duration: | 1.1.2022 – 31.12.2024 |
| Self assembly and functionalization of nanofibrillar DNA-spider silk hybrid materials | |
| Self assembly and functionalization of nanofibrillar DNA-spider silk hybrid materials | |
| Program: | Inter-academic agreement |
| Project leader: | RNDr., Ing. Šipošová Katarína, PhD. |
| Duration: | 1.1.2022 – 31.12.2023 |
| NOVÉ KOMPOZITY NANOČASTÍC OXIDU CÉRIA A UHLÍKOVÝCH ENTEROSORBENTOV PRE LIEČBU CHORÔB PO AKÚTNOM OŽIARENÍ | |
| NOVEL COMPOSITES BASED ON CERIUM OXIDE NANOPARTICLES AND CARBON ENTEROSORBENTS FOR ACUTE RADIATION SICKNESS THERAPY | |
| Program: | NATO |
| Project leader: | MUDr. Musatov Andrey, DrSc. |
| Duration: | 15.7.2020 – 14.7.2023 |
| Zvýšenie Bioaktivity Nanočastíc Oxidu Céria | |
| Enhancement of Bioactivity of Cerium Oxide Nanoparticles | |
| Program: | Inter-academic agreement |
| Project leader: | MUDr. Musatov Andrey, DrSc. |
| Duration: | 1.1.2020 – 31.12.2021 |
| MAGBBRIS – Nové magnetické biomateriály pre obnovu mozgu a zobrazovanie po mozgovej príhode | |
| New MAGnetic Biomaterials for Brain Repair and Imaging after Stroke | |
| Program: | ERANET |
| Project leader: | doc. RNDr. Kopčanský Peter, CSc. |
| Annotation: | By engineering novel magnetic nano-biomaterials we will achieve tissue repair in the context of an ischemic event. We will take advantage of nanotechnology to deliver therapeutic growth factors, secreted by progenitor cells, into the injured brain.According to the World Health Organization, 15 million persons suffer a stroke worldwide eachyear. However, the only available treatment is the acute thrombolytic therapy (pharmacological or mechanical) which is being administered to less than 10% of stroke patients due to strict selectioncriteria. In contrast, neuro-repair treatments could offer the opportunity to include most strokepatients by extending the therapeutic time window.MAGBBRIS will demonstrate that growth factors, secreted by endothelial progenitor cells, with proved potential to induce tissue repair, can be encapsulated in magnetic biomaterials and be successfully and safely transplanted into mouse brains to induce tissue repair. In the ischemic brain, the secretome will be retained by an external magnetic field in the vasculature, improving vascular remodelling and neurogenic tissue regeneration after stroke. |
| Duration: | 1.3.2018 – 28.2.2021 |
| AMAZON – Dynamické štúdium amyloidnej agregácie proteínov pomocou magnetických zeolitových nanočastíc | |
| Dynamical study of formation/destruction of protein amyloid aggregatess targeted by magnetic zeolite nanocomposites | |
| Program: | JRP |
| Project leader: | doc. RNDr. Kopčanský Peter, CSc. |
| Annotation: | Abnormal protein aggregation and accumulation of formed fibrils are characteristic features for a range of, if not all, neurodegenerative disorders such as Alzheimer’s, Huntington’s, Parkinson’s, as well as non-neuropathic amyloidosis. Nanoparticles (NPs), attributed to its particularities in sizes, chemical composition and surface properties, have already been found effective in influencing amyloid fibrils. Our preliminary results acquired on amyloid fibrils incubated with in-laboratory synthesized Fe3O4 nanoparticles (MNPs) indicated reductive potential of MNPs on formation of amyloid fibrils, and also suggested facilitation of inhibiting preformation and eradication of amyloid fibrils may be plausible when the fibrils are exposed to external radiation in presence of MNPs. Also, with our previously reported photoluminescence properties of natural zeolite (CZ) which is a promising material for biomedicine and pharmaceutics due to its non-toxicity, thermal stability, expanded surface area, and exceptional ability to adsorb various atoms, organic molecules and nanoparticles into micro- and mesopores, we plan to apply the MNPs with CZ, developing multiphoton excitation microscopy (MPEM), and other appropriate instruments to establish the dynamical investigation of amyloid fibril formation and remodeling in real time. Prior to in vivo experiment, cytotoxicity in different type of cells and animal models such as zebrafish and mice will be evaluated. |
| Duration: | 1.1.2018 – 31.12.2020 |
| SOOSA – Štúdium usporiadania, orientácie a samozbaľovania v biopolyméroch a kvapalných kryštáloch | |
| Study of orientation ordering and self-assembly in biopolymer and liquid crystal | |
| Program: | Bilateral – other |
| Project leader: | RNDr., Ing. Šipošová Katarína, PhD. |
| Annotation: | Liquid crystals (LCs) are matter in a state which has properties between those of conventional liquids and those of solid crystals. Examples of liquid crystals can be found both in the natural world and in technological applications. LCs actually exhibit a plethora of unique and attractive properties that offer tremendous potential for fundamental science as well as innovative applications depending on ordering properties of anisotropic molecules at interfaces in systems. In particular, new bio-nanocomposites consisting of protein amyloid fibrils (that can be consider as long and rigid objects similarly copolymers due to their stability and rigidity) and magnetic nanoparticles provide achievement of high special order and alignment. Within the project, we propose to focus on the effects of backbone-alignment on the ordering and self-assembly in these systems. |
| Duration: | 1.1.2018 – 31.12.2019 |
| MACOSYS – Magneticky aktívne anizotrópne kompozitne systémy | |
| Magnetically active anisotropic composite systems | |
| Program: | ERANET |
| Project leader: | doc. RNDr. Kopčanský Peter, CSc. |
| Annotation: | The proposal relates to basic research of anisotropic soft materials sensitive to magnetic fields. The targeted substances are composite materials, so called ferronematics (nematic liquid crystals (LCs) doped with spherical, rod-like, chain-like magnetite nanoparticles, or with carbon nanotubes functionalized with magnetite nanoparticles), and the cross-linked liquid crystalline polymers (elastomers) doped with nanoparticles. The key objectives are: (i) to measure the optical and dielectric responses of these materials to low magnetic fields; (ii) to explore which conditions influence these responses (e.g., bias magnetic field, pretilt, anchoring between the liquid crystal and nanoparticles); (iii) to contribute to the better understanding of the major problem in ferronematics (FNs): the aggregation process; (iv) to enhance the magnetic field induced phase transition temperature shift in a novel type of FNs; (v) to produce mesogenic cross-linked composites sensitive to magnetic fields. |
| Duration: | 1.9.2013 – 31.8.2016 |
| Amyloidná agregácia proteínov na hybridných povrchoch | |
| Amyloid aggregation of proteins in hybrid interfaces | |
| Program: | Inter-academic agreement |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Annotation: | We are interested to study the effect of nano-confinement on structure, stability and other properties of proteins leading to formation of amyloid structures. Poly/peptides behave differently on surfaces, interfaces or small length scales compared to their bulk properties. Understanding such differences is crucial in many applications where proteins are constrained in nanometer size spaces. The results will provide new insights into the effects of soft-matter confinement on protein amyloid fibrillation, a situation usually met in natural cell environments. |
| Duration: | 1.1.2013 – 31.12.2015 |
| AMYTOX – Štúdium amyloidogénnych proteínov a ich cytotoxicity | |
| Investigation of the amyloidogenic proteins in relationship with their cytotoxic effect | |
| Program: | Bilateral – other |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Annotation: | The project offers us the possibility to take part into international collaboration with Romanian Scientists concern to amyloid aggregation of proteins. Moreover, the proposal of the project will enable mutual utilization of equipment provided by both institutions. At the same time the young members of Slovakian team will have the opportunity to learn new techniques in well-equipped laboratories at UMF „Carol Davila“ and work in the international scientific team.The topic of the project includes the research of the protein self-assembly into amyloidal aggregates, since this characteristic is one of the hallmarks of multiple severe diseases(Alzheimer’s disease, non-neuropathic amyloidosis). Although multiple studies in the research field of amyloidosis were carry out, the data on the mechanism formation or reverse of the process of the amyloidosis are missing, and the toxicity of amyloid aggregates remains to be clarified. Our attention will be addressed to assess the conditions required for promotion of protein missfolding, to determine the cytotoxic effect of the amyloids, and to identify molecules able to inhibit protein aggregation. The major advantages of the bilateral cooperation will consist in: i) the study could provide a useful model for understanding the pathological protein aggregation and their cytotoxic action; ii) the scientific output of the project could suggest possible alternatives of therapy against devastating diseases, as different types of dementia or non-neuropathic amyloidosis; iii) the experimental data will offer thepossibility to publish our joint results in the international scientific journals; iv) our collaboration could represent a solid base to apply further for EU funding projects. |
| Duration: | 1.1.2013 – 31.12.2014 |
| LYSACELL – Štúdium amyloidnej agregácie lyzozýmu in vitro a analýza vplyvu agregácie na prežívanie buniek | |
| Investigation of the lysozyme amyloid aggregtiion using in vitro assays and analysing its effects on cell viability and proliferation | |
| Program: | Bilateral – other |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Annotation: | The topic of the project includes the research of the protein self-assembly into amyloidal aggregates, since this characteristic is one of the hallmarks of multiple severe diseases. Amyloidosis is a disorder of protein folding in which normally soluble proteins undergo conformational changes and are deposited in an abnormal fibrillar form. For instance, the presence of tau protein deposits is associated with neurodegenerative diseases, like Alzheimer\’s disease and the presence of lysozyme with systemic amyloidosis.One of the objectives of the project is characterization of the conditions leading to protein conformational changes which promote amyloid aggregation. Also, the attention will be focused to investigate the effect of amyloid assemblies (soluble and insoluble) on viability and proliferation of the cells and to analyse their effects on the cell cycle phases and apoptotic processes. The significant spotlight will be given to identify effective molecules or molecular complexes involved in inhibition or destruction of amyloid polymerization and to examine their cytotoxicity. |
| Duration: | 3.1.2011 – 31.12.2012 |
National
| Štúdium a modifikácia vlastností pavúčieho proteínu nadprodukovaného v Escherichia coli | |
| – | |
| Program: | VEGA |
| Project leader: | RNDr., Ing. Šipošová Katarína, PhD. |
| Duration: | 1.1.2022 – 31.12.2025 |
| Vývoj translačne relevantných regeneračných a reparatívnych stratégií po traumatickom poranení miechy | |
| The development of translationally relevant regenerative and reparative strategies after spinal cord trauma | |
| Program: | SRDA |
| Project leader: | MUDr. Musatov Andrey, DrSc. |
| Duration: | 1.7.2020 – 30.6.2024 |
| Nový pohľad na vplyv hydrofóbnych interakcií na tvorbu a stabilitu proteínových agregátov. Prepojenie na oxidačný stres. | |
| New Insight into the Role of Hydrophobic Interactions in Formation and Stability of Proteins Aggregates. Link to Oxidative Stress. | |
| Program: | VEGA |
| Project leader: | MUDr. Musatov Andrey, DrSc. |
| Duration: | 1.1.2021 – 31.12.2023 |
| MVISION – Nanočastice v anizotrópnych systémoch | |
| Nanoparticles in anisotropic systems | |
| Program: | SRDA |
| Project leader: | doc. RNDr. Kopčanský Peter, CSc. |
| Annotation: | The proposal is devoted to study complex anisotropic systems based on thermotropic as well as lyotropic(biological) liquid crystals. Liquid crystals represents the uniq state of matter, which is liquid but posses theanisotropic properties. The structuralization phenomena in such systems play key role in fundamental as well asin applied research. The main aim is to s influence the sensitivity of these anisotropic systems to externalmagnetic field, what will be done by adding suitable magnetic nanoparticles and open the way for theirapplications in magneto-optical devices. |
| Duration: | 1.7.2016 – 31.12.2020 |
| Oxidačný stres a fosfolipidovo-proteínové interakcie: funkčné a štrukturálne dôsledky | |
| Functional and Structural Insights into the Phospholipid-Protein Interaction during Oxidative Stress | |
| Program: | VEGA |
| Project leader: | MUDr. Musatov Andrey, DrSc. |
| Annotation: | Mitochondria play a critical role in cells metabolism and mitochondrial dysfunction has long been implicated in age-related neurodegenerative diseases. One currently accepted theory is that damage to mitochondria, the main source of reactive oxygen species (ROS), initiates these diseases. In fact, when ROS levels overpower the cell’s defenses (oxidative stress), proteins, nucleic acids and/or lipids are irreversibly damaged. Particularly important is that oxidative stress disrupts or modifies the protein-lipid interactions what is one of the essential features of normal cell operation. Such disruptions could be a decisive factor leading to ROS-induced diseases. To test this hypothesis and clearly elucidate the links between mitochondrial oxidative stress and cells dysfunction we propose to investigate both, (i) the role of phospholipid modification in ROS-induced damage to mitochondrial electron transport Complex IV, and (ii) the role of phospholipids and oxidatively modified phospholipids in amyloidogenesis. |
| Duration: | 1.1.2017 – 31.12.2020 |
| – | |
| Inhibitors of protein amyloid aggregation | |
| Program: | VEGA |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Annotation: | Amyloid aggregation is on high interest due to its impact on properties of poly/peptides that are arranged into highly organized amyloid aggregates. Amyloids play an important role in serious diseases (type II diabetes, Alzheimer’s diseases) and impose serious restriction in pharmaceutical utilization of proteins. Although multiple studies were carrying out, the data on the mechanism formation or reverse of amyloidosis are missing. The project is devoted to contribute to the understanding of the process of amyloid aggregation and to identify the active substances (small molecules and nanoparticles) that reduce the amyloid self-assembly of proteins providing a basis for the development of drugs for the treatment of amyloid pathology. We will determine the correlation between the properties of active substances and their anti-amyloid activity. We will identify binding sites for the active compounds and suggest the mechanism binding of active molecules using experimental and mathematical methods. |
| Duration: | 1.1.2013 – 31.12.2016 |
| METAMYLC – Štrukturalizačné javy v systémoch s nanočasticami | |
| Structuralization phenomena in systems with nanoparticles | |
| Program: | SRDA |
| Project leader: | doc. RNDr. Kopčanský Peter, CSc. |
| Annotation: | The structuralization phenomena plays key role in fundamental as well as in applied research. The proposed project is devoted to the study of the influence of nanoparticles on the structuralization phenomena in various systems as well as on the structuralization of the nanoparticles alone. Some special kinds of nanoparticles will be prepared, which will be used for study these effects. The main aim is to study the influence of the nanoparticles on the structuralization phenomena in two different kind of systems as liquid crystalline mater and amyloid structures. In the first system the magnetic particles can increase the sensitivity of liquid crystals on an external magnetic field. In the amyloid structures the nanoparticles can significantly influence the amyloid aggregation of proteins, which is responsible for amyloid diseases as Alzheimher, Parkinson and Dibetes II The prepared nanoparticles will also be used to build their 3D structures (metamaterials), i.e. to create systems with unusual physical properties as for example negative reflection. |
| Duration: | 1.5.2011 – 31.10.2014 |
| Amyloidná agregácia proteínov | |
| Amyloid aggregation of proteins | |
| Program: | VEGA |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Annotation: | Amyloid aggregation of proteins is characteristic of several human pathologies termed amyloid diseases (diabetes type II, Alzheimer\’s and prion diseases) and imposes serious restriction in pharmaceutical and biotechnological utilization of proteins. The proposed project is oriented to the characterization of intramolecular and intermolecular interactions of non-native protein conformers leading to self-assembly of molecules into amyloid aggregates with the aim to determine some common mechanisms required for formation of amyloid structures. The significant focus is given to identification of active substances (low molecular compounds, nanoparticles, molecular complexes) effective in reducing of the amount of amyloid aggregates providing a basis for the development of drugs for the treatment of amyloid pathology. |
| Duration: | 1.1.2010 – 31.12.2012 |