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| Iónové kvapaliny a hlboko eutektické zmesi ako modulátory stability a agregácie proteínov | |
| – | |
| Program: | VEGA |
| Project leader: | RNDr. Fedunová Diana, PhD. |
| Duration: | 1.1.2022 – 31.12.2025 |
| Objasnenie počiatočných štádií amyloidnej agregácie proteínov – od mechanizmu k terapii | |
| Unraveling the early events of protein amyloid aggregation – from mechanism to therapy | |
| Program: | VEGA |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Annotation: | Structural changes of poly/peptides leading to the formation of amyloid aggregates are associated with incurablediseases, like Alzheimer\’s disease or diabetes. While the general mechanisms of fibril formation and theircharacterization are well reported, the early events during poly/peptide fibrillation are still unclear. The project isfocused on understanding the early events mechanisms leading to the formation of pre-fibrillar (partially un/foldedintermediates, nuclei, oligomers) and fibrillar amyloid aggregates of selected globular and intrinsically disorderedproteins. Our focus will be the study of the kinetics of pre-fibrillar structures formation, their morphology, andcytotoxicity, under various experimental conditions, and in the presence of selected interacting partners (smallmolecules, nanoparticles). The obtained results will contribute to understanding the early events of amyloidaggregation and identifying the inhibitors with therapeutic potential for amyloid diseases. |
| Duration: | 1.1.2021 – 31.12.2024 |
| MULTIHIT – Multifunkčné inhibítory poly/peptidov spojených s Alzheimerovou chorobou | |
| Multi-target inhibitors of poly/peptides associated with Alzheimer´s disease | |
| Program: | SRDA |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Duration: | 1.7.2019 – 1.6.2023 |
| Nové metódy v štatistickej a korelačnej analýze parametrických modelov povrchov a ich distribúcií | |
| Novel statistical and correlation methods in analysis of parametric models of surfaces and their distributions. | |
| Program: | VEGA |
| Project leader: | RNDr. Marek Jozef, PhD. |
| Duration: | 1.1.2019 – 31.12.2021 |
| Samousporiadanie poly/peptidov do amyloidných agregátov – mechanizmus, inhibícia a cytotoxicita | |
| Self-assembly of poly/peptides into amyloid aggregates – mechanism, inhibition and cytotoxicity | |
| Program: | VEGA |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Annotation: | Amyloid supramolecular complexes formed by poly/peptides are the most prevalent naturally occurring self-assembling systems. Formation of such complexes affects function of poly/peptides and is associated with more than 30 serious amyloid-related diseases such as Alzhemer’s disease or diabetes mellitus. The exactmechanism of amyloid self-assembly of poly/peptides is not known yet and no effective treatment of amyloidosis is established. The goal of the project is to study the mechanism of the amyloid aggregation of poly/petides with different native structures and identification of inhibitors of poly/peptide self-assembly since the inhibition of amyloid formation is one of the possible therapeutic approaches against amyloid-related diseases. We will focus on the determination of the correlation between the morphology of amyloid aggregates and their cytotoxicity as well as on the relationship between structure of the effective inhibitors and their anti-amyloid activity. |
| Duration: | 1.1.2017 – 31.12.2020 |
| – | |
| Inhibitors of protein amyloid aggregation | |
| Program: | VEGA |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Annotation: | Amyloid aggregation is on high interest due to its impact on properties of poly/peptides that are arranged into highly organized amyloid aggregates. Amyloids play an important role in serious diseases (type II diabetes, Alzheimer’s diseases) and impose serious restriction in pharmaceutical utilization of proteins. Although multiple studies were carrying out, the data on the mechanism formation or reverse of amyloidosis are missing. The project is devoted to contribute to the understanding of the process of amyloid aggregation and to identify the active substances (small molecules and nanoparticles) that reduce the amyloid self-assembly of proteins providing a basis for the development of drugs for the treatment of amyloid pathology. We will determine the correlation between the properties of active substances and their anti-amyloid activity. We will identify binding sites for the active compounds and suggest the mechanism binding of active molecules using experimental and mathematical methods. |
| Duration: | 1.1.2013 – 31.12.2016 |
| Vývoj a implementácia algoritmov a metód na štúdium vláknitých objektov použitím spracovania obrazu a matematického modelovania | |
| Development and implementation of algorithms and methods for the study of fiber like objects using image processing and mathematical modeling | |
| Program: | VEGA |
| Project leader: | Ing. Demjén Erna, PhD. |
| Annotation: | The goal of the project is to develop methods and algorithms for the study of fiber-like objects. Fiber-like objects are thin, elongated structures approximately of circular cross section that can touch each other, intersect each other and create clusters. Artificial simulated images along with real experimental images (e.g. amyloid fibrils scanned by atomic force microscope) will be studied by image processing, machine learning and mathematical modeling techniques. |
| Duration: | 1.1.2014 – 31.12.2016 |
| MICRO_NUI – Interaktívny zber a spracovanie obrazov v mikroskopii použitím prirodzeného užívateľského rozhrania | |
| Interactive methods of image acquisition and processing in microscopy using natural user interface | |
| Program: | SRDA |
| Project leader: | doc. Ing. Tomori Zoltán, CSc. |
| Annotation: | Communication with computers via the natural user interface (NUI) has growing importance in several areas. Our goal is the utilization of specific NUI devices, e.g. Microsoft Kinect, touch tablet and the brain potentials sensor to control the „state of the art" experimental devices such as optical tweezers and optical scalpel. The project is aimed to propose new and modify existing algorithms in the field of computer vision and image analysis, that allow not only recognition of microscopic particles, but also automatic or semi-automatic manipulation of them. The part of this process is appropriate visualization in mono or stereo mode that represents a feedback of interactive algorithms. |
| Duration: | 1.7.2012 – 31.12.2015 |
| Interaktívne algoritmy spracovania obrazu založené na minimalizácii energetickej funkcie a metóde "Graph-cuts". | |
| Interactive Image Processing Algorithms Based on Energy Minimization and "Graph-cuts" Method | |
| Program: | VEGA |
| Project leader: | doc. Ing. Tomori Zoltán, CSc. |
| Annotation: | The goal of the project is to extend interactive capabilities of Graph-cuts algorithm. Our aim is to supply user with a set of tools represented as cursors of various shapes that would be automatically adapted according to properties of underlying image. This method will be tested on segmentation of individual pictures as well on off-line analysis of video records of moving objects. The modified version of this algorithm should perform 3D reconstruction based on the series of images capturing an object under different angles of view using the planned prototype for Molecular Fluorescence Tomography. |
| Duration: | 1.1.2011 – 31.12.2013 |
| Amyloidná agregácia proteínov | |
| Amyloid aggregation of proteins | |
| Program: | VEGA |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Annotation: | Amyloid aggregation of proteins is characteristic of several human pathologies termed amyloid diseases (diabetes type II, Alzheimer\’s and prion diseases) and imposes serious restriction in pharmaceutical and biotechnological utilization of proteins. The proposed project is oriented to the characterization of intramolecular and intermolecular interactions of non-native protein conformers leading to self-assembly of molecules into amyloid aggregates with the aim to determine some common mechanisms required for formation of amyloid structures. The significant focus is given to identification of active substances (low molecular compounds, nanoparticles, molecular complexes) effective in reducing of the amount of amyloid aggregates providing a basis for the development of drugs for the treatment of amyloid pathology. |
| Duration: | 1.1.2010 – 31.12.2012 |
| Analýza fluorescenčného obrazu nepravidelných buniek s cieľom nedeštruktívnej kvantifikácie DNA | |
| Fluorescent image analysis of irregularly shaped cells for purposes of non-destructive DNA contents quantification | |
| Program: | SRDA |
| Project leader: | doc. Ing. Tomori Zoltán, CSc. |
| Annotation: | Current non-destructive methods of DNA contents measurement in living cells are based either on densitometric analysis of static fluorescent images or on the dynamic acquisition of signal when a cell flows under the detector of flow cytometer. Both methods failed in irregularly shaped cells like e.g. sperm cells.Proposed project is based on the combination of both approaches that means controlled rotation of an immobilized sperm cell in a transparent capillary and acquisition of emitted light by CCD camera. The series of images representing the sperm cell under different angles of view leads to the design of a 3D model which allows comparison of flatten objects acquired under different conditions which make DNA measurement more objective. Automation of the experiment is based on the analysis of many immobilized sperm cells localized in the capillary which allows exploitation of such apparatus in the assisted reproduction centers where they could replace expensive and not very precise flow cytometer. This is a way how to avoid the using of defected sperm causing a transfer of genetic diseases. |
| Duration: | 1.6.2008 – 31.12.2010 |
| Metódy segmentácie postupnosti obrazov pomocou aktívnych kontúr a ich využitie v biomedicíne | |
| Methods of images sequence segmentation using active contours and their exploitation in biomedicine | |
| Program: | VEGA |
| Project leader: | doc. Ing. Tomori Zoltán, CSc. |
| Annotation: | The project solves the problem of the segmentation of image sequences representing either serial opticalsections obtained by a confocal microscope or time sequences of images obtained by a video camera. The mainmethod in our approach in segmentation is the active contour model. Our own modification is based on the wireconductor model and is proposed in this project. The algorithms of the segmentation will be tested on thefollowing applications: 3D reconstruction of microvessels net in the brain, determination of the chromosomesnumber in a sperm based on its shape analysis and mobility monitoring of minipigs suffering from Huntingtondisease. All these applications have the direct link to existing projects solved at cooperating institutions |
| Duration: | 1.1.2008 – 31.12.2010 |
| Agregácia proteínov a identifikácia inhibítorov agregácie | |
| Protein aggregation and identification of aggregation inhibitors | |
| Program: | VEGA |
| Project leader: | doc. RNDr. Gažová Zuzana, DrSc. |
| Annotation: | The project is focused to obtain new knowledge of the aspects of protein misfolding and protein aggregation as the formation of protein deposits is one of the hallmarks of many very serious diseases and seems to be one of the most important problem in biomedicine\’s and biotechnological utilization of proteins. Project deals with investigation of relationship between various conformational states of proteins and their propensity to aggregate. The disease related protein (lysozyme, tau) as well as model protein system (cytochrome c, myoglobin, polyamino acids) will be used for this purpose. The significant focus will be given to identify inhibitors of protein aggregation. We will searching especially for low molecular compounds. |
| Duration: | 1.1.2007 – 31.12.2009 |