Pand\u00e9mia COVID-19 uk\u00e1zala, ak\u00fa d\u00f4le\u017eit\u00fa \u00falohu zohr\u00e1va r\u00fdchla a spo\u013eahliv\u00e1 diagnostika pri odha\u013eovan\u00ed a monitorovan\u00ed infek\u010dn\u00fdch ochoren\u00ed. Nukleov\u00e9 kyseliny, vr\u00e1tane RNA, s\u00fa d\u00f4le\u017eit\u00fdmi biomarkermi pou\u017e\u00edvan\u00fdmi pri detekcii, monitorovan\u00ed a lie\u010dbe \u0161irokej \u0161k\u00e1ly chor\u00f4b. Ich \u00fa\u010dinn\u00e1 izol\u00e1cia z\u00a0biologick\u00fdch vzoriek je v\u0161ak n\u00e1ro\u010dn\u00e1 najm\u00e4 v pr\u00edpadoch, ke\u010f sa vyskytuj\u00fa vo ve\u013emi n\u00edzkych koncentr\u00e1ci\u00e1ch.<\/p>\n
Vedci z n\u00e1\u0161ho \u00fastavu (I. Antal, A. Anto\u0161ov\u00e1, V. Z\u00e1vi\u0161ov\u00e1, M. Kubov\u010d\u00edkov\u00e1, A. Jur\u00edkov\u00e1, P. Pill\u00e1rova, V. Jedin\u00e1k, Z. Ga\u017eov\u00e1, M. Konerack\u00e1<\/strong>) sk\u00famali inovat\u00edvny pr\u00edstup zalo\u017een\u00fd na magnetick\u00fdch nano\u010dasticiach pre \u00fa\u010dinnej\u0161iu izol\u00e1ciu RNA. Ich v\u00fdsledky boli publikovan\u00e9 v \u010dasopise Next Materials<\/em><\/strong><\/span>.<\/p>\n V\u00fdskumn\u00fd t\u00edm sa zameral na magnetick\u00e9 nano\u010dastice pokryt\u00e9 vrstvou oxidu kremi\u010dit\u00e9ho (SiO2<\/sub>@MNPs) a sk\u00famal, ako by sa dali upravi\u0165 ich povrchov\u00e9 vlastnosti, aby sa zlep\u0161ila ich interakcia s nukleov\u00fdmi kyselinami. Za t\u00fdmto \u00fa\u010delom v\u00fdskumn\u00edci modifikovali povrch t\u00fdchto SiO2<\/sub>@MNPs dvoma kladne nabit\u00fdmi biopolym\u00e9rmi \u2013 chitoz\u00e1nom \u00a0a poly-L-lyz\u00ednom.<\/p>\n Princ\u00edp tohto pr\u00edstupu je jednoduch\u00fd: nukleov\u00e9 kyseliny nes\u00fa za experiment\u00e1lnych podmienok z\u00e1porn\u00fd n\u00e1boj, zatia\u013e \u010do polym\u00e9rom obalen\u00e9 SiO2<\/sub>@MNPs s\u00fa kladne nabit\u00e9, \u010do podporuje ich interakciu s RNA. Magnetick\u00e9 jadro z\u00e1rove\u0148 umo\u017e\u0148uje r\u00fdchlu separ\u00e1ciu \u010dast\u00edc zo vzorky pomocou vonkaj\u0161ieho magnetick\u00e9ho po\u013ea.<\/p>\n Vedci z\u00a0\u00daEF SAV podrobne charakterizovali modifikovan\u00e9 \u010dastice z h\u013eadiska ich ve\u013ekosti, tvaru, povrchov\u00e9ho n\u00e1boja, magnetick\u00fdch vlastnost\u00ed a \u010fal\u0161\u00edch relevantn\u00fdch charakterist\u00edk. Z\u00edskan\u00e9 v\u00fdsledky im umo\u017enili ur\u010di\u0165 podmienky, za ktor\u00fdch chitoz\u00e1n a poly-L-lyz\u00edn vytvorili na magnetick\u00fdch \u010dasticiach \u00fa\u010dinn\u00fa povrchov\u00fa vrstvu.<\/p>\n Najv\u00fdznamnej\u0161\u00edm v\u00fdsledkom danej \u0161t\u00fadie je v\u00fdrazn\u00e1 RNA separa\u010dn\u00e1 schopnos\u0165 novo pripraven\u00fdch nano\u010dast\u00edc. Nano\u010dastice modifikovan\u00e9 chitoz\u00e1nom vyextrahovali 58,64 % RNA, zatia\u013e \u010do pri nano\u010dasticiach s\u00a0povrchom modifikovan\u00fdm poly-L-lyz\u00ednom bola \u00fa\u010dinnos\u0165 33,99 %. Pre porovnanie, p\u00f4vodn\u00e9 SiO2<\/sub>@MNPs nano\u010dastice s\u00a0negat\u00edvne nabit\u00fdm povrchom vyextrahovali za rovnak\u00fdch podmienok len 3,86 % RNA.<\/p>\n Tieto v\u00fdsledky ukazuj\u00fa, \u017ee modifik\u00e1cia magnetick\u00fdch \u010dast\u00edc kladne nabitou organickou vrstvou m\u00f4\u017ee v\u00fdrazne zlep\u0161i\u0165 ich schopnos\u0165 viaza\u0165 a izolova\u0165 RNA. Z\u00e1rove\u0148 sa uk\u00e1zalo, \u017ee \u00fa\u010dinnos\u0165 izol\u00e1cie v\u00fdznamne z\u00e1vis\u00ed od pou\u017eit\u00e9ho povrchov\u00e9ho polym\u00e9ru. Nano\u010dastice modifikovan\u00e9 chitoz\u00e1nom dosiahli v porovnan\u00ed s poly-L-lyz\u00ednom v\u00fdrazne vy\u0161\u0161iu separa\u010dn\u00fa \u00fa\u010dinnos\u0165, \u010do poukazuje na v\u00fdznam vhodnej vo\u013eby povrchovej \u00fapravy pri v\u00fdvoji materi\u00e1lov ur\u010den\u00fdch na izol\u00e1ciu nukleov\u00fdch kysel\u00edn.<\/p>\n V\u00fdsledky tejto \u0161t\u00fadie potvrdzuj\u00fa potenci\u00e1l povrchovo funkcionalizovan\u00fdch magnetick\u00fdch nano\u010dast\u00edc ako \u00fa\u010dinn\u00e9ho n\u00e1stroja na izol\u00e1ciu nukleov\u00fdch kysel\u00edn. \u010eal\u0161\u00ed v\u00fdvoj t\u00fdchto materi\u00e1lov by mohol vies\u0165 k r\u00fdchlej\u0161\u00edm a efekt\u00edvnej\u0161\u00edm met\u00f3dam izol\u00e1cie RNA z komplexn\u00fdch biologick\u00fdch vzoriek, s mo\u017en\u00fdm vyu\u017eit\u00edm v biomedic\u00ednskom v\u00fdskume a diagnostick\u00fdch technol\u00f3gi\u00e1ch.<\/p>\n <\/p>\n Text, foto: M. Konerack\u00e1<\/p>\n","protected":false},"excerpt":{"rendered":" Pand\u00e9mia COVID-19 uk\u00e1zala, ak\u00fa d\u00f4le\u017eit\u00fa \u00falohu zohr\u00e1va r\u00fdchla a spo\u013eahliv\u00e1 diagnostika pri odha\u013eovan\u00ed a monitorovan\u00ed infek\u010dn\u00fdch ochoren\u00ed. Nukleov\u00e9 kyseliny, vr\u00e1tane RNA, s\u00fa d\u00f4le\u017eit\u00fdmi biomarkermi pou\u017e\u00edvan\u00fdmi…<\/p>\n","protected":false},"author":11,"featured_media":10250,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-10249","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-aktuality"],"_links":{"self":[{"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/posts\/10249","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/comments?post=10249"}],"version-history":[{"count":3,"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/posts\/10249\/revisions"}],"predecessor-version":[{"id":10253,"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/posts\/10249\/revisions\/10253"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/media\/10250"}],"wp:attachment":[{"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/media?parent=10249"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/categories?post=10249"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/websrv.saske.sk\/uef\/wp-json\/wp\/v2\/tags?post=10249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}